Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Aptamers: Current Applications in Leukemia Diagnostics and Therapeutics.

Nucleic acid therapeutics·2026

Same author

Translational opportunities in aptamer and nanobody lateral flow assays within the WHO REASSURED framework.

Sensors & diagnostics·2026

Same author

Prevention and prediction of in-hospital delirium: The case for biomarker-guided treatment and prophylaxis.

General hospital psychiatry·2026

Same author

The 10-23 DNAzyme in Biosensing and Diagnostics: Applications, Challenges, and Future Directions.

Angewandte Chemie (International ed. in English)·2026

Same author

Transforming RNA Degradation into Accurate RNA Detection: RNase I-Assisted Rolling Circle Amplification for Clinical Samples.

Angewandte Chemie (International ed. in English)·2026

Same author

A modified X10-23 DNAzyme that can better access large, structured RNA targets.

Nucleic acids research·2026

Same journal

Fluorescent merocyanines: from fundamental properties to applications as molecular probes, in bioimaging and as emissive dye aggregates.

Chemical Society reviews·2026

Same journal

Direct impure water electrolysis at industrial scale.

Chemical Society reviews·2026

Same journal

Catalytic valorization of polyolefins: from catalysts and processes to reactors.

Chemical Society reviews·2026

Same journal

Designing stable π-radicals.

Chemical Society reviews·2026

Same journal

Antibacterial drug discovery: challenges and preclinical promises from synthetic small molecules.

Chemical Society reviews·2026

Same journal

Selective carbon-carbon bond cleavage involving alkene moieties.

Chemical Society reviews·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 29, 2025

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

Biosensing with DNAzymes.

Erin M McConnell¹, Ioana Cozma, Quanbing Mou

¹Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8S 4K1, Canada. liying@mcmaster.ca.

Chemical Society Reviews

|July 6, 2021

Summary

This summary is machine-generated.

DNAzymes, or DNA enzymes, offer versatile biosensing capabilities for portable diagnostics. This review highlights their progress in molecular recognition for point-of-care applications.

More Related Videos

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Detection of Bacteria Using Fluorogenic DNAzymes

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Related Experiment Videos

Last Updated: Oct 29, 2025

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Detection of Bacteria Using Fluorogenic DNAzymes

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Area of Science:

Biochemistry
Molecular Biology
Biotechnology

Background:

Functional nucleic acids, particularly DNAzymes, are catalytic DNA molecules with diverse applications.
DNAzymes have emerged as promising tools in biosensing due to their stability and programmability.
The integration of DNAzymes into bioassays is crucial for developing novel diagnostic platforms.

Purpose of the Study:

To provide a comprehensive review of biosensing applications utilizing DNAzymes.
To highlight significant advancements in DNAzyme-based portable biosensors and point-of-care diagnostics.
To offer a critical perspective on the future impact of DNAzyme technology.

Main Methods:

Review of existing literature on DNAzyme-based biosensing.
Analysis of recent progress in in vivo sensing platforms and portable sensor development.
Discussion of molecular recognition strategies employing DNAzymes.

Main Results:

DNAzymes have demonstrated significant progress in various sensing applications.
Advancements include the development of in vivo sensing platforms and portable DNAzyme sensors.
Key contributions focus on enhancing molecular recognition for diagnostics.

Conclusions:

DNAzyme-based biosensors show great potential for point-of-care diagnostics and portable devices.
Further development is expected to broaden the impact of DNAzyme technology in healthcare.
DNAzymes represent a powerful tool for molecular recognition in advanced biosensing.