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CircCSNK1G1 Contributes to the Tumorigenesis of Gastric Cancer by Sponging miR-758 and Regulating ZNF217 Expression

Feng Qiang1, Jingjing Li1

  • 1Department of Gastroenterology, the First People's Hospital of Huzhou, Huzhou, 313000, People's Republic of China.

Cancer Management and Research
|July 8, 2021
PubMed
Abstract

Insights

Circular RNAs (circRNAs) regulate cancer. This study shows circCSNK1G1 accelerates gastric cancer (GC) by sponging miR-758, upregulating ZNF217, indicating circCSNK1G1 is a potential GC biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are increasingly recognized as key regulators in diverse cancers.
  • The specific role of circCSNK1G1 in gastric cancer (GC) remains largely unexplored.

Purpose of the Study:

  • To investigate the function and mechanism of circCSNK1G1 in gastric cancer progression.
  • To explore the potential of circCSNK1G1 as a diagnostic and therapeutic target for GC.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) and Western blotting were used to assess gene and protein expression.
  • In vitro assays (Transwell, CCK-8, flow cytometry) and in vivo xenograft assays evaluated GC cell behavior.
  • RNA immunoprecipitation (RIP) and luciferase reporter assays confirmed molecular interactions.

Main Results:

  • circCSNK1G1 and ZNF217 were upregulated, while miR-758 was downregulated in GC tissues and cells.
  • circCSNK1G1 depletion inhibited GC progression both in vitro and in vivo.
  • circCSNK1G1 acts as a sponge for miR-758, leading to increased ZNF217 expression and promoting GC development.

Conclusions:

  • circCSNK1G1 accelerates gastric cancer progression through the miR-758/ZNF217 signaling axis.
  • circCSNK1G1 presents a promising potential biomarker for GC diagnosis and therapeutic intervention.
Keywords:
GCZNF217circCSNK1G1gastric cancermiR-758

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