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Biochemical Methods to Analyze the Subcellular Localization of NF-κB Proteins Using Cell Fractionation

Davide Vecchiotti1, Daniela Verzella1, Daria Capece1

  • 1Department of Biotechnological and Applied Clinical Sciences (DISCAB), University of L'Aquila, L'Aquila, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|July 8, 2021
PubMed

Keywords:
CentrifugationCytoplasmatic proteinNF-κBNuclear proteinSucrose

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Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
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Differential centrifugation is...
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