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Targeting improved reproducibility in surface-enhanced Raman spectroscopy with planar substrates using 3D printed

Buddini Iroshika Karawdeniya1, Robert B Chevalier2, Y M Nuwan D Y Bandara3

  • 1Department of Electronic Materials Engineering, Research School of Physics, The Australian National University, Canberra ACT 2601, Australia.

The Review of Scientific Instruments
|July 10, 2021
PubMed
Summary
This summary is machine-generated.

Drop-casting for surface-enhanced Raman spectroscopy (SERS) can be unreliable. Using 3D printed holders to control sample placement significantly improves SERS measurement reproducibility and signal intensity.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Materials Science

Background:

  • Drop-casting is a common method for sample deposition in surface-enhanced Raman spectroscopy (SERS).
  • Solvent evaporation during drop-casting can lead to inhomogeneous analyte distribution on the substrate.
  • Inconsistent sample distribution can compromise SERS measurement accuracy and reproducibility.

Purpose of the Study:

  • To develop and evaluate 3D printed holders for precise spatial control of drop-cast samples in SERS.
  • To investigate the impact of controlled spatial offsets on SERS signal intensity and reproducibility.

Main Methods:

  • Fabrication of 3D printed holders designed for spatial registry of drop-cast sample centers.
  • Systematic variation of the spatial offset between the analyte droplet center and the laser excitation spot (0-900 µm).
  • Performance evaluation of the 3D printed holders using SERS measurements.

Main Results:

  • The 3D printed holders enabled controlled spatial positioning of the dried sample droplet center.
  • Deliberate spatial offsets prevented significant signal intensity drops (up to ~3x) typically observed with central drop placement.
  • Improved measurement reproducibility was achieved by offsetting the excitation laser from the droplet center.

Conclusions:

  • 3D printed holders offer a simple, cost-effective solution to enhance the reliability of SERS measurements using drop-casting.
  • Controlled spatial offsets are crucial for mitigating signal variations caused by inhomogeneous sample distribution.
  • This approach improves the practical utility of drop-casting for SERS analysis, especially with limited sample volumes.