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A multispectral-sensor-based colorimetric reader for biological assays.

M Pirbhai1, C Albrecht2, C Tirrell1

  • 1Department of Physics, St. Lawrence University, 23 Romoda Dr., Canton, New York 13617, USA.

The Review of Scientific Instruments
|July 10, 2021
PubMed
Summary
This summary is machine-generated.

A new colorimetric reader simplifies biosensing for labs. This device uses a multispectral sensor and Raspberry Pi to accurately detect color changes, improving glucose detection limits.

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Area of Science:

  • Biosensing and analytical chemistry
  • Instrumentation and device development

Background:

  • Colorimetric assays are widely used in biosensing.
  • Existing methods may require complex calibration or image processing.
  • Resource-limited laboratories need accessible and user-friendly instrumentation.

Purpose of the Study:

  • To develop a simple, low-cost colorimetric reader for biosensing applications.
  • To demonstrate the device's utility in a relevant assay.
  • To evaluate its performance compared to existing methods.

Main Methods:

  • A commercial multispectral sensor was interfaced with a Raspberry Pi and touchscreen.
  • The device was applied to a birefringent enzyme-linked immunosorbent assay (ELISA).
  • Performance was assessed by its ability to prevent false positives and determine the limit of detection.

Main Results:

  • The reader requires no user calibration of wavelengths or extensive image processing.
  • It successfully prevented certain false positives in the ELISA.
  • The limit of detection for glucose was reduced by an order of magnitude.

Conclusions:

  • The developed colorimetric reader is simple, cost-effective, and easy to operate.
  • It offers significant advantages for resource-limited research settings.
  • The device enhances the performance of colorimetric biosensing assays, such as ELISA.