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Related Experiment Video

Updated: Oct 29, 2025

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
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Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

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RNAi and Ino80 complex control rate limiting translocation step that moves rDNA to eroding telomeres.

Manasi S Apte1, Hirohisa Masuda2, David Lee Wheeler1

  • 1Laboratory of Biochemistry and Molecular Biology, NCI, NIH, Bethesda, MD 20892, USA.

Nucleic Acids Research
|July 10, 2021
PubMed
Summary
This summary is machine-generated.

Telomere-free chromosome ends form via ribosomal DNA (rDNA) translocation, requiring RNAi and Ino80 complex. These pathways also regulate subtype choice, revealing crosstalk in unusual translocation reactions.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Chromosomal Biology

Background:

  • Canonical telomeres protect chromosome ends, but alternative mechanisms exist.
  • HAATIrDNA represents a telomerase-negative survival mode using ribosomal DNA (rDNA) repeats at chromosome ends.

Purpose of the Study:

  • To elucidate the mechanism of HAATIrDNA formation and the roles of RNAi and Ino80C.
  • To investigate the relationship between RNAi and Ino80C in controlling translocation events.

Main Methods:

  • Genetic analysis of translocation events.
  • Investigating the requirement for RNAi and Ino80C in HAATIrDNA formation.
  • Characterizing the dual roles of Dcr1 and Iec1 in HAATI subtype choice.

Main Results:

  • HAATIrDNA formation initiates with a single translocation of rDNA from Chr III to subtelomeric elements (STE) on Chr I or II, dependent on RNAi and Ino80C.
  • The STE-rDNA junction is then propagated to other STE chromosome ends independently of RNAi or Ino80C.
  • Dcr1 (RNAi) and Iec1 (Ino80C) exhibit dual roles, promoting HAATIrDNA but inhibiting the rare HAATISTE subtype.

Conclusions:

  • RNAi and Ino80C are crucial for initiating HAATIrDNA formation through a specific translocation event.
  • These pathways exhibit crosstalk, with components playing dual roles in regulating different HAATI subtypes.
  • This study reveals an unappreciated interplay between RNAi and Ino80C in managing unusual chromosomal end formations.