Multilocus sequence typing analysis and second-generation sequencing analysis of Salmonella Wandsworth

Area of Science:

• Microbiology
• Genomics
• Bacterial Pathogenesis

Background:

• Salmonella Wandsworth is a rare serotype.
• Limited information exists on its genetic makeup and virulence.
• This study addresses this knowledge gap through comprehensive genomic analysis.

Purpose of the Study:

• To perform genotyping and genome structure analysis of Salmonella Wandsworth.
• To investigate the molecular biological functions of this rare serotype.
• To understand its relationship with other Salmonella strains.

Main Methods:

• Serological typing and antibiotic susceptibility testing (micro broth dilution).
• Multilocus Sequence Typing (MLST) for homology analysis.
• Whole-genome sequencing and bioinformatic analyses (COG, KEGG, virulence factors, CRISPR-Cas).

Main Results:

• Salmonella Wandsworth identified as Group Q, MLST ST1498.
• Demonstrated sensitivity to ceftriaxone, imipenem, chloramphenicol, colistin; resistance to ampicillin, cefalotin, gentamicin, ciprofloxacin.
• Genome analysis revealed 5,109,457 bp length, 3,746 COG genes, 1,340 KEGG genes involved in 19 metabolic pathways, 249 virulence factors, 2 disease islands, and identified the aminoglycoside resistance gene aac(6')-Iaa.

Conclusions:

• Salmonella Wandsworth is confirmed as Salmonella Group Q.
• Further research and continuous monitoring of clinical infections are essential.
• Genomic insights aid in understanding its pathogenic potential and evolution.