Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Blood-brain Barrier

The Blood-brain Barrier

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

New Synthetic Strategies Toward DFO*: Enhanced Yield and Purity of a Key Chelator for <sup>89</sup>Zr Chemistry.

Pharmaceuticals (Basel, Switzerland)·2026

Same author

Dissecting the propensity of RIM1 subdomains to form phase condensates.

Scientific reports·2026

Same author

Adalimumab-polyelectrolyte nanocomplexes decelerate synovial drug clearance <i>ex vivo</i>.

Nanoscale·2026

Same author

A multi-level characterization of TfR, Mfsd2a and LRP1 in a stem cell derived in vitro model of the blood-brain barrier.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2026

Same author

Spray-freeze-drying aprepitant with hydroxypropyl cellulose increases nasal bioavailability.

International journal of pharmaceutics: X·2026

Same author

Balancing Stealth and Targetability: cCPP- and PEG-Modified Liposomes for the Targeted Delivery of Anti-HER2 Nanobodies.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

Silicone oil in protein drug products and its implications for formulation stability.

Advanced drug delivery reviews·2026

Same journal

Targeted delivery of proteolysis-targeting chimeras (PROTAC) and molecular glue degraders (MGD).

Advanced drug delivery reviews·2026

Same journal

Lysosome-targeting degrader delivery system: from formulation design to biomedical applications.

Advanced drug delivery reviews·2026

Same journal

Anti-PEG antibodies in nanomedicine: Mechanisms, risks, and opportunities.

Advanced drug delivery reviews·2026

Same journal

Optimizing macrophage-targeted intracellular delivery systems for safe and effective immunotherapies.

Advanced drug delivery reviews·2026

Same journal

Light-controlled CRISPR-dCas9 epigenome editing: advanced drug-delivery strategies and oncology applications.

Advanced drug delivery reviews·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 29, 2025

A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

Published on: April 9, 2019

Blood-brain barrier models: Rationale for selection.

Amira Sayed Hanafy¹, Dirk Dietrich², Gert Fricker³

¹Department of Pharmaceutics, Institute of Pharmacy, University of Bonn, Bonn, Germany; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt.

Advanced Drug Delivery Reviews

|July 11, 2021

Summary

This summary is machine-generated.

Selecting appropriate blood-brain barrier (BBB) models is crucial for successful brain drug delivery research. This review provides a rationale for choosing the right BBB model to improve preclinical to clinical translation.

Keywords:

Animal modeling Brain targeting Cerebral capillaries Drug delivery In vivo-in vitro correlation Nanotechnology

More Related Videos

Setting-up an In Vitro Model of Rat Blood-brain Barrier BBB: A Focus on BBB Impermeability and Receptor-mediated Transport

Setting-up an In Vitro Model of Rat Blood-brain Barrier BBB: A Focus on BBB Impermeability and Receptor-mediated Transport

Published on: June 28, 2014

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells

Published on: September 24, 2017

Related Experiment Videos

Last Updated: Oct 29, 2025

A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

Published on: April 9, 2019

Setting-up an In Vitro Model of Rat Blood-brain Barrier BBB: A Focus on BBB Impermeability and Receptor-mediated Transport

Setting-up an In Vitro Model of Rat Blood-brain Barrier BBB: A Focus on BBB Impermeability and Receptor-mediated Transport

Published on: June 28, 2014

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells

Published on: September 24, 2017

Area of Science:

Neuroscience
Pharmacology
Biomedical Engineering

Background:

The blood-brain barrier (BBB) presents a significant challenge for brain drug delivery due to its limited permeability.
BBB complexity and its role in neurological diseases are increasingly understood.
Numerous drug delivery systems fail in clinical trials, often attributed to inadequate preclinical models.

Purpose of the Study:

To provide a rationale for selecting appropriate blood-brain barrier (BBB) models for drug delivery research.
To discuss essential considerations for optimizing BBB model selection.
To improve the reliability of preclinical data and enhance clinical trial success rates.

Main Methods:

Review of existing literature on BBB models (in vitro, in vivo, ex vivo, in situ, in silico).
Analysis of factors influencing BBB permeability and drug transport.
Discussion of the impact of model selection on research outcomes.

Main Results:

Inadequate BBB model selection significantly impacts preclinical data reliability and clinical trial outcomes.
A systematic approach to model selection based on research questions and applications is needed.
Key considerations for optimal BBB model selection are identified.

Conclusions:

The choice of BBB model is critical for the success of brain drug delivery research.
A well-defined rationale for BBB model selection can bridge the gap between preclinical findings and clinical efficacy.
Optimizing BBB model selection is essential for advancing therapeutic strategies for brain diseases.