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Tagging and Fusion Proteins

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Related Experiment Video

Updated: Oct 29, 2025

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Published on: April 22, 2022

Simplified Epigenome Profiling Using Antibody-tethered Tagmentation.

Steven Henikoff^1,2, Jorja G Henikoff¹, Kami Ahmad¹

¹Basic Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, Seattle, Washington 98109, USA.

|July 12, 2021

Summary

This summary is machine-generated.

Cleavage Under Targets & Tagmentation (CUT&Tag) now maps chromatin accessibility alongside epigenomic profiling. This simplified method uses accessible DNA sites for high-resolution mapping in single tubes, suitable for any lab.

Keywords:

CUT&Tag Chromatin accessibility Epigenomic profiling Histone modifications RNA polymerase II

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Last Updated: Oct 29, 2025

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TChIP-Seq: Cell-Type-Specific Epigenome Profiling

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Published on: January 23, 2019

Area of Science:

Epigenetics
Molecular Biology
Genomics

Background:

Antibody-mediated tethering of Tn5 transposase enables epigenomic profiling via CUT&Tag.
This method maps chromatin features in small samples and single cells.
Previous CUT&Tag protocols focused on specific chromatin marks.

Purpose of the Study:

To adapt CUT&Tag for simultaneous chromatin profiling and accessibility mapping.
To develop a simplified, high-resolution method for mapping chromatin accessibility.
To make epigenomic profiling accessible for various laboratory settings.

Main Methods:

Permeabilization of cells or nuclei followed by antibody addition.
Incubation with a Protein A-Transposase fusion protein.
Modification of transposase activation conditions to target accessible DNA sites for tagmentation.

Main Results:

CUT&Tag successfully mapped chromatin accessibility using specific histone and RNA Polymerase II antibodies.
The adapted method achieved high signal-to-noise ratios and resolution in accessibility maps.
All library preparation steps were streamlined into single PCR tubes.

Conclusions:

CUT&Tag can be modified to simultaneously profile chromatin features and map accessibility.
The simplified protocol is robust, cost-effective, and suitable for diverse research environments.
This advancement offers a versatile tool for epigenomic research.