Euzebya pacifica sp. nov., a novel member of the class Nitriliruptoria

A Gram-stain-positive, aerobic, chemo-organotrophic, rod-shaped, non-spore-forming strain, which produced convex, circular, pink-pigmented colonies, designated as DY32-46^T, was isolated from seawater collected from the Pacific Ocean. DY32-46^T was found to grow at 20-40 °C (optimum, 30-35 °C), pH 6.0-8.0 (optimum, pH 6.5) and with 0-5 % (w/v) NaCl (optimum, 1-2 %). The results of chemotaxonomic analysis indicated that the respiratory quinone of DY32-46^T was MK-9(H₄), and major fatty acids (>10 %) were C_17 : 1 ω8c, summed feature 3 (C_16 : 1 ω7c and/or C_16 : 1 ω6c), C_16 : 0 and C_15 : 1 ω6c. The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, one unidentified aminophospholipid, three unidentified glycolipids, three unidentified phospholipids, one unidentified phosphoglycolipid and five unidentified lipids. The DNA G+C content of DY32-46^T was 70.6 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences and genomic data indicated that DY32-46^T should be assigned to the genus Euzebya. ANI and in silico DNA-DNA hybridization values between strain DY32-46^T and type strains of Euzebya species were 73.1-87.2 % and 20.2-32.4 %, respectively. Different phenotypic properties, together with genetic distinctiveness, demonstrated that strain DY32-46^T was clearly distinct from recognized species of the genus Euzebya. Therefore, DY32-46^T represents a novel species within the genus Euzebya, for which the name Euzebya pacifica sp. nov is proposed. The type strain is DY32-46^T (=MCCC 1K03476^T=KCTC 49091^T).