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Updated: Oct 29, 2025

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
New strategy for detection of hydrogen peroxide based on bi-nucleophilic reaction
Chenxin Mao1, Yafei Tian2, Shuoshuo Wang1
1Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
Abstract:
Two novel fluorescent probes based on 7-hydroxy-4-methyl-coumarin, FAA-MC-OH (2-fluoro-4-nitro-phenylacetyl hydroxyl coumarin) and FBA-MC-OH (2-fluoro-4-nitro-benzoyl hydroxyl coumarin) are first synthesized, and spectral studies confirm that both the probes display highly selective and sensitive to H2O2, especially FBA-MC-OH has a shorter response time. Moreover, it is worth noting that the reaction mechanism is based on bi-nucleophilic substitution instead of oxidation or hydrolysis, which is different from previous reported probes'.
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