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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
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Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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iPS Cell Differentiation01:22

iPS Cell Differentiation

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The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
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Stem Cell Culture01:17

Stem Cell Culture

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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Related Experiment Video

Updated: Oct 29, 2025

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
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Bioengineering platforms for cell therapeutics derived from pluripotent and direct reprogramming.

Yoonhee Jin1, Seung-Woo Cho

  • 1Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.

APL Bioengineering
|July 14, 2021
PubMed
Summary

Cellular reprogramming and tissue engineering advance regenerative medicine, overcoming clinical hurdles for cell therapies. These combined approaches enhance cell function and create novel engineered tissues for repair.

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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
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Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Area of Science:

  • Regenerative Medicine
  • Biomedical Engineering
  • Cellular Biology

Background:

  • Pluripotent and direct reprogramming technologies are key to tissue repair and organ function restoration.
  • Induced pluripotent stem cells and directly reprogrammed cells represent significant advancements in regenerative medicine.
  • Clinical application of these cell-based therapies faces obstacles that need to be addressed.

Purpose of the Study:

  • To explore the synergistic potential of cellular reprogramming and tissue engineering for advancing regenerative medicine.
  • To identify how bioengineering technologies can enhance the efficacy of cell therapeutics derived from reprogramming.
  • To outline how combining reprogramming with tissue engineering can overcome current limitations in cell-based therapies.

Main Methods:

  • Utilizing induced pluripotent stem cells and direct reprogramming techniques.
  • Implementing bioengineering platforms including biomaterials, bioprinting, microfluidic devices, and biostimulatory systems.
  • Investigating the enhancement of cell viability, differentiation, and function through these combined approaches.

Main Results:

  • Bioengineering technologies significantly improve cell viability, differentiation, and function.
  • The integration of reprogramming with tissue engineering enhances the efficacy of cell therapeutics.
  • New methods for producing engineered tissue substitutes are being developed.

Conclusions:

  • Cellular reprogramming combined with tissue engineering offers a powerful strategy to overcome bottlenecks in cell-based therapies.
  • These integrated approaches are poised to revolutionize tissue repair and restoration of organ function.
  • The development of engineered tissue substitutes holds great promise for future clinical applications.