Label-Free Fluorescent Aptasensor for Adenosine Triphosphate Detection Using SYBR Gold as a Probe

Area of Science:

• Bioanalytical Science
• Molecular Biology
• Biochemistry

Background:

• Adenosine triphosphate (ATP) is a crucial molecule in biological processes.
• Accurate detection of ATP is vital for various research applications.
• Existing ATP detection methods may lack selectivity or require complex procedures.

Purpose of the Study:

• To develop a label-free sensing strategy for sensitive and selective ATP detection.
• To create a cost-effective and rapid aptasensor for ATP quantification.
• To validate the aptasensor's performance in complex biological matrices.

Main Methods:

• Utilized aptamers that undergo conformational changes upon ATP binding.
• Employed exonuclease I for selective digestion of unfolded aptamers.
• Incorporated SYBR Gold as a fluorescent probe to enhance signal detection.
• Developed a label-free aptasensor for adenosine triphosphate (ATP) detection.

Main Results:

• Achieved high selectivity for ATP detection.
• Established a low limit of detection (39.2 nM) for ATP.
• Successfully detected ATP in human serum samples with high recovery rates (93.8%-108.1%).
• Demonstrated effective ATP monitoring in cell extracts without interference.

Conclusions:

• The developed aptasensor offers a promising approach for ATP detection in bioanalytical science.
• The method is cost-effective, rapid, easy to use, and highly selective.
• The aptasensor is suitable for practical applications in biological and biomedical research.