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Draft Genome Sequences of the Bap-Producing Strain Staphylococcus aureus V329 and Its Derived Phage-Resistant Mutant

Lucía Fernández1,2, Ana Catarina Duarte1,2, Beatriz Martínez1,2

  • 1Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Asturias, Spain.

Microbiology Resource Announcements
|July 15, 2021
PubMed

Insights

Researchers sequenced the genomes of Staphylococcus aureus V329 and a phage-resistant mutant. A point mutation in the tagO gene was identified in the mutant strain, providing insights into phage resistance mechanisms in bovine mastitis pathogens.

Area of Science:

  • Microbiology
  • Genomics
  • Veterinary Science

Background:

  • Staphylococcus aureus is a significant pathogen causing bovine mastitis.
  • Bovine mastitis leads to substantial economic losses in the dairy industry.
  • Bovine-associated Staphylococcus aureus strains can possess virulence factors like biofilm-associated protein (Bap).

Purpose of the Study:

  • To perform draft genome sequencing of Staphylococcus aureus V329, a Bap-producing strain from bovine mastitis.
  • To sequence the genome of a derived mutant strain (BIM-1) exhibiting resistance to phage phiIPLA-RODI.
  • To identify genetic differences contributing to phage resistance in Staphylococcus aureus.

Main Methods:

  • Whole-genome sequencing of the wild-type and mutant Staphylococcus aureus strains.
  • Comparative genomic analysis to identify genetic variations between the two strains.
  • Bioinformatic analysis to pinpoint mutations in specific genes.

Main Results:

  • Draft genome sequences for Staphylococcus aureus V329 and its phage-resistant mutant BIM-1 were obtained.
  • Comparative analysis revealed a single point mutation in the tagO gene of the BIM-1 mutant strain.
  • The tagO gene is implicated in the development of resistance to phage phiIPLA-RODI.

Conclusions:

  • The tagO gene plays a crucial role in Staphylococcus aureus resistance to specific bacteriophages.
  • Genome sequencing is a valuable tool for understanding the genetic basis of phage resistance in mastitis pathogens.
  • Findings contribute to the development of phage-based strategies for controlling Staphylococcus aureus in dairy herds.

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