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Methodological Considerations for Lipid and Polar Component Analyses in Human Skin Stratum Corneum
E Berdyshev1, I Bronova2, D Y M Leung2
1National Jewish Health, 1400 Jackson Street, Goodman Building, K924b, Denver, CO, 80206, USA. berdysheve@njhealth.org.
Cell Biochemistry and Biophysics
|July 15, 2021
Summary
Tape stripping and mass spectrometry analyze skin
Area of Science:
- Dermatology and allergy research
- Biochemistry and molecular analysis
Background:
- Stratum corneum analysis via tape stripping and mass spectrometry offers advantages for allergy research.
- Challenges exist in analyzing diverse molecules from minimal skin tape strip samples.
Purpose of the Study:
- To review current knowledge on skin lipids and polar molecules.
- To discuss challenges in processing skin tape strip samples.
- To guide approaches for complex, quantitative stratum corneum analysis in clinical trials.
Main Methods:
- Review of existing literature on stratum corneum analysis.
- Discussion of mass spectrometry techniques for molecular analysis.
- Exploration of sample preparation methods for minimal skin samples.
Main Results:
- Overview of lipid and polar molecule composition in the skin.
- Identification of key challenges in analyzing tape-stripped stratum corneum.
- Guidance on generating normalized, quantitative proteomic data.
Conclusions:
- Optimized methods are crucial for analyzing diverse molecules from skin tape strips.
- Quantitative, normalized data are essential for robust clinical trial analysis of allergic skin diseases.
