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Updated: Oct 28, 2025

Detection of Protease Activity by Fluorescent Peptide Zymography
Published on: January 20, 2019
Analysis of Murein Hydrolases and Proteases Through Zymography
Nichole A Seawell1, Jeffrey L Bose2
1Department of Microbiology, Molecular Genetics and Immunology, The University of Kansas Medical Center, Kansas City, KS, USA.
Abstract:
Zymography has been used to analyze enzymatic activity and processing of enzymes for many years. We have used bacterial cells copolymerized into the acrylamide gel to analyze specific activity of murein hydrolases of interest. In addition, this method has been widely used to examine and distinguish protease activities using different substrates. This chapter provides instruction for zymography of both extracellular murein hydrolases and proteases produced by Staphylococcus aureus.

