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Published on: April 18, 2019
Evaluation of Staphylococcus aureus Antibiotic Tolerance Using Kill Curve Assays
Jessica N Brandwein1, Kelly C Rice2
1Department of Microbiology and Cell Science, IFAS, University of Florida, Gainesville, FL, USA.
Abstract:
This chapter describes the use of antibiotic kill curves to examine the tolerance of Staphylococcus aureus to any antibiotic of interest. This is done by treating cultures with a super-minimum inhibitory concentration (MIC) of antibiotic and measuring viability over time by colony-forming units (CFUs). Kill curves provide a unique insight into S. aureus antibiotic tolerance and death patterns that may not be clear from other experiments, such as traditional MIC or Kirby-Bauer assays.

