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Updated: Oct 28, 2025

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
Published on: June 14, 2020
Parallel processing of working memory and temporal information by distinct types of cortical projection neurons
Jung Won Bae1, Huijeong Jeong1,2, Young Ju Yoon1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea.
Abstract:
It is unclear how different types of cortical projection neurons work together to support diverse cortical functions. We examined the discharge characteristics and inactivation effects of intratelencephalic (IT) and pyramidal tract (PT) neurons-two major types of cortical excitatory neurons that project to cortical and subcortical structures, respectively-in the deep layer of the medial prefrontal cortex in mice performing a delayed response task. We found stronger target-dependent firing of IT than PT neurons during the delay period. We also found the inactivation of IT neurons, but not PT neurons, impairs behavioral performance. In contrast, PT neurons carry more temporal information than IT neurons during the delay period. Our results indicate a division of labor between IT and PT projection neurons in the prefrontal cortex for the maintenance of working memory and for tracking the passage of time, respectively.
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