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Factor X-activating activity in patients with colorectal carcinoma
R Dover1, N L Goeting, I Taylor
1University Surgical Unit, Southampton General Hospital, UK.
The British Journal of Surgery
|December 1, 1987
Summary
Colorectal carcinomas exhibit significantly higher factor X-activating activity (FXAA) than normal adjacent tissue. This increased procoagulant activity in tumors was observed in 76% of patients studied.
Area of Science:
- Biochemistry
- Oncology
- Hematology
Background:
- Blood coagulation factor X plays a role in hemostasis.
- Alterations in coagulation factor activity may be associated with cancer development.
- Colorectal cancer is a significant global health concern.
Purpose of the Study:
- To investigate the direct activation of blood coagulation factor X by malignant colorectal tissue.
- To compare factor X-activating activity (FXAA) in tumor tissue versus adjacent normal colonic mucosa.
- To assess the correlation between FXAA levels and clinicopathological features of colorectal carcinoma.
Main Methods:
- Homogenization of resected colorectal tissues (malignant and normal) and non-malignant bowel disease tissue.
- Incubation with purified factor X and calcium ions.
- Spectrophotometric measurement of activated factor X generation using a chromogenic substrate.
Main Results:
- Factor X-activating activity (FXAA) was detected in all tested tissues.
- Tumor homogenates showed significantly greater FXAA compared to adjacent normal colonic mucosa (P < 0.001).
- A tumor:normal FXAA ratio > 1.2 was observed in 76% of colorectal cancer patients; no correlation with tumor differentiation, stage, or site was found.
Conclusions:
- Colorectal carcinomas possess significantly higher FXAA than adjacent non-malignant colonic mucosa.
- The study did not find direct evidence linking procoagulant levels to the extent of malignancy in these patients.
- FXAA in normal colonic mucosa from cancer patients is comparable to that in patients with non-malignant bowel disease.