A simple and rapid fluorescent approach for Pb²⁺ determination and application in water samples and living cells

A novel selective fluorescent chemosensor, thiosemicarbazide-appended naphthalimide derivative (TND), has been designed and synthesized, which exhibited good selectivity and sensibility for Pb²⁺ in CH₃CN:H₂O (1:1) solution. The probe TND showed obvious color changes under UV light of 365 nm and displayed turn-on fluorescence response with Pb²⁺ added. The binding mode of TND with Pb²⁺ was found to be 1:1 based on the Job's plot analysis. The detection limit of Pb²⁺ was 4.7 nM, which is far below the allowable concentration determined by WHO in drinking water. Moreover, the fortified recoveries of Pb²⁺ were from 100.54% to 113.68% in water samples. TND is also applied for fluorescence imaging of Pb²⁺ in lysosomes of human stromal cell line (HSC). This study indicated that TND would be a potential sensor detecting Pb²⁺ in real sample.