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Separation of sialyl-oligosaccharides by medium pressure anion-exchange chromatography on Mono Q
J van Pelt1, J B Damm, J P Kamerling
1Department of Bio-Organic Chemistry, Transitorium III, Utrecht University, The Netherlands.
Carbohydrate Research
|November 15, 1987
Abstract:
On columns prepacked with the recently introduced anion-exchange material, Mono Q (Pharmacia), sialyl-oligosaccharides could be fractionated excellently according to the sialic acid content. With u.v. absorption at 214 nm as the detection method, analytical runs of carbohydrate material on the microgram scale, were possible. The value of the method for preparative purposes was demonstrated for sialic acid-containing carbohydrates obtained from human serotransferrin by hydrazinolysis.