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Updated: Oct 28, 2025
The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
Published on: September 27, 2016
Sensitive Detection of Single-Nucleotide Polymorphisms by Solid Nanopores Integrated With DNA Probed Nanoparticles
Ling Zhi Wu1,2, Yuan Ye1, Zhi Xuan Wang2
1Key Laboratory for Organic Electronics and Information Displays, Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials, National Synergetic Innovation Center for Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing, China.
A new nanopore sensing method detects single-nucleotide polymorphisms (SNPs) with high sensitivity. This breakthrough in genetic variation detection holds promise for early cancer diagnosis and disease prevention.
Area of Science:
- Biotechnology
- Nanotechnology
- Genetics
Background:
- Single-nucleotide polymorphisms (SNPs) are common genetic variations linked to diseases like cancer.
- Accurate detection of SNPs is crucial for understanding disease mechanisms and developing diagnostics.
Purpose of the Study:
- To develop a novel assay for single-nucleotide discrimination using nanopore sensing.
- To detect specific SNPs associated with gastric cancer using a new biosensing strategy.
Main Methods:
- Utilized a nanopore sensing platform with DNA-probed gold nanoparticles as carriers.
- Employed single-nucleotide discrimination for detecting p53 gene mutations in gastric cancer.
Main Results:
- Successfully detected p53 gene mutation SNPs in gastric cancer at femtomolar concentrations.
- Demonstrated high sensitivity and spatial resolution in single-nucleotide distinction.
Conclusions:
- The developed robust biosensing strategy enables precise SNP detection.
- Nanopore sensing integrated with nanoparticles shows significant potential for early disease diagnosis and prevention.
