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Updated: Oct 27, 2025
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Influence of a High-Impact Multidimensional Rehabilitation Program on the Gut Microbiota of Patients with Multiple
Monica Barone1, Laura Mendozzi2, Federica D'Amico1
1Department of Medical and Surgical Sciences, University of Bologna, 40138 Bologna, Italy.
Abstract:
Multiple sclerosis (MS) is a neurodegenerative inflammatory condition mediated by autoreactive immune processes. Due to its potential to influence host immunity and gut-brain communication, the gut microbiota has been suggested to be involved in the onset and progression of MS. To date, there is no definitive cure for MS, and rehabilitation programs are of the utmost importance, especially in the later stages. However, only a few people generally participate due to poor support, knowledge, and motivation, and no information is available on gut microbiota changes. Herein we evaluated the potential of a brief high-impact multidimensional rehabilitation program (B-HIPE) in a leisure environment to affect the gut microbiota, mitigate MS symptoms and improve quality of life. B-HIPE resulted in modulation of the MS-typical dysbiosis, with reduced levels of pathobionts and the replenishment of beneficial short-chain fatty acid producers. This partial recovery of a eubiotic profile could help counteract the inflammatory tone typically observed in MS, as supported by reduced circulating lipopolysaccharide levels and decreased populations of pro-inflammatory lymphocytes. Improved physical performance and fatigue relief were also found. Our findings pave the way for integrating clinical practice with holistic approaches to mitigate MS symptoms and improve patients' quality of life.
Insights
A brief high-impact multidimensional rehabilitation program (B-HIPE) positively modulated the gut microbiota in multiple sclerosis (MS) patients. This intervention reduced harmful bacteria, increased beneficial microbes, and improved quality of life.
Area of Science:
- Neuroimmunology
- Microbiome Research
- Rehabilitation Science
Background:
- Multiple sclerosis (MS) is a chronic inflammatory disease affecting the central nervous system.
- The gut microbiota's role in MS pathogenesis and progression is increasingly recognized.
- Current rehabilitation strategies for MS often lack engagement and fail to address the gut-brain axis.
Purpose of the Study:
- To investigate the impact of a brief high-impact multidimensional rehabilitation program (B-HIPE) on gut microbiota composition in MS patients.
- To assess the effects of B-HIPE on MS symptoms, inflammatory markers, and quality of life.
- To explore the potential of integrating holistic, leisure-based rehabilitation with gut health management for MS.
Main Methods:
- Participants with MS underwent a brief high-impact multidimensional rehabilitation program (B-HIPE) in a leisure setting.
- Gut microbiota composition was analyzed pre- and post-intervention, focusing on microbial profiles and metabolite producers.
- Clinical assessments included physical performance, fatigue levels, circulating inflammatory markers (e.g., lipopolysaccharide), and quality of life questionnaires.
Main Results:
- B-HIPE significantly modulated the gut microbiota, reducing pathobionts and increasing beneficial short-chain fatty acid (SCFA)-producing bacteria, moving towards a eubiotic state.
- A decrease in circulating lipopolysaccharide levels and pro-inflammatory lymphocytes was observed, suggesting a reduced inflammatory tone.
- Participants reported improvements in physical performance and significant relief from fatigue, alongside enhanced quality of life.
Conclusions:
- A brief, multidimensional rehabilitation program can positively influence the gut microbiota in individuals with MS.
- Modulation of gut dysbiosis and reduction in inflammatory markers may contribute to symptom improvement and enhanced quality of life in MS.
- This study supports the integration of holistic, leisure-based rehabilitation approaches targeting the gut-brain axis for comprehensive MS management.
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