"Biomethanation of syngas by enriched mixed anaerobic consortium in pressurized agitated column"

In a circular economy approach, heterogeneous wastes can be upgraded to energy in the form of syngas via pyrogasification, and then to methane via biomethanation. Working at high pressure is a promising approach to intensify the process and to reduce gas-liquid transfer limitations. However, raising the pressure could lead to reaching the CO inhibition threshold of the microorganisms involved in syngas-biomethanation. To investigate the impact on pressure on the process, a 10L continuous stirred tank reactor working at 4 bars and 55 °C was implemented. Syngas (40% CO, 40% H₂, 20% CO₂) biomethanation was performed successfully and methane productivity as high as 6.8 mmol_CH4/L_reactor/h with almost full conversion of CO (97%) and H₂ (98%) was achieved. CO inhibition was investigated and carboxydotrophs appeared less resistant to high CO exposition than methanogens.