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Related Experiment Video

Updated: Oct 27, 2025

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types

Published on: May 10, 2016

Multi-omics approach highlights differences between RLP classes in Arabidopsis thaliana.

C E Steidele¹, R Stam²

¹Chair of Phytopathology, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Emil-Ramann- Straße 2, 85354, Freising, Germany.

|July 21, 2021

Summary

Researchers classified Leucine rich-repeat receptor-like proteins (RLPs) of unknown function in Arabidopsis thaliana. Key differences in abundance and genomic clustering distinguish defense-related RLPs from those involved in basal functions.

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Last Updated: Oct 27, 2025

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types

Published on: May 10, 2016

Lateral Root Inducible System in Arabidopsis and Maize

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

Area of Science:

Plant molecular biology
Genomics
Proteomics

Background:

The Leucine rich-repeat receptor-like protein (RLP) family in Arabidopsis thaliana comprises 57 members.
RLPs play roles in plant development and defense, but functions of many remain unknown.

Purpose of the Study:

To functionally annotate Arabidopsis RLPs with unknown functions.
To identify distinguishing characteristics between defense-related and basal function RLPs.

Main Methods:

Analysis of publicly available transcriptome and proteome datasets.
Genome-wide annotation of RLPs based on functional categories.
Comparative analysis of RLP subclasses for genomic clustering and genetic diversity.

Main Results:

RLPs were categorized as defense-related or basal function.
Significant differences were observed in transcript/protein abundance and genomic clustering between subclasses.
No significant difference in genetic diversity was found between the RLP subclasses.

Conclusions:

Functional annotation of unknown RLPs aids in identifying potential immune receptors.
Nucleotide diversity alone is insufficient to differentiate defense vs. developmental RLPs.
Transcript/protein abundance and genomic clustering offer insights into RLP function across plant species.