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Related Experiment Video

Updated: Oct 27, 2025

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Published on: March 8, 2024

A method for constructing GLP-1 overexpression intestinal organoids.

Zhi-Yang Zeng¹, Jia-Wei Lu¹, Xi-Ya Cao¹

¹Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, China.

Yi Chuan = Hereditas

|July 21, 2021

Summary

Researchers developed GLP-1 overexpressing mouse intestinal organoids. These organoids enhanced glucose tolerance in mice, offering a potential new treatment strategy for type 2 diabetes.

Keywords:

GLP-1 intestinal organoids type 2 diabetes

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Last Updated: Oct 27, 2025

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A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids

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Published on: April 20, 2015

Area of Science:

Endocrinology and Metabolism
Gastroenterology
Regenerative Medicine

Background:

Glucagon-like peptide 1 (GLP-1) is a key hormone regulating blood glucose by stimulating insulin release.
GLP-1 analogs are promising for type 2 diabetes treatment, but novel delivery strategies are needed.
Intestinal organoids offer a model for studying gut hormone function and developing therapeutic approaches.

Purpose of the Study:

To construct mouse intestinal organoids engineered to overexpress glucagon-like peptide 1 (GLP-1).
To evaluate the therapeutic potential of GLP-1 overexpressing organoids in improving glucose homeostasis.
To establish a novel in vitro model for investigating GLP-1-based diabetes therapies.

Main Methods:

Optimized lentivirus infection to achieve GLP-1 overexpression in mouse intestinal organoids.
Cultured GLP-1 overexpressing organoids and collected their secreted supernatants.
Administered organoid supernatants to wild-type and diabetic mice to assess effects on glucose tolerance.

Main Results:

Successfully generated mouse intestinal organoids with enhanced GLP-1 expression.
Supernatants from GLP-1 overexpressing organoids significantly improved glucose tolerance in both wild-type and diabetic mouse models.
Demonstrated the biological activity and therapeutic potential of the engineered organoids.

Conclusions:

Engineered GLP-1 overexpressing intestinal organoids represent a viable novel strategy for type 2 diabetes treatment.
This approach may offer a new avenue for cell-based or secreted factor-based therapies for metabolic disorders.
The study provides a foundation for further development of organoid-based therapeutic systems for diabetes.