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Related Experiment Video

Updated: Oct 27, 2025

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Lin28 paralogs regulate lung branching morphogenesis.

Jihan K Osborne1, Melissa A Kinney1, Areum Han1

  • 1Division of Pediatric Hematology/Oncology, Boston Children's Hospital Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.

Cell Reports
|July 21, 2021
PubMed
Summary
This summary is machine-generated.

Lin28a and Lin28b proteins regulate early lung development by controlling messenger RNA (mRNA) and microRNA (miRNA) processing. Their coordinated action is essential for normal branching morphogenesis, preventing developmental defects.

Keywords:
Lin28a/Lin28bbranching morphogenesislung developmentpost-transcriptional regulation

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Organ development relies on precisely timed molecular events.
  • Branching morphogenesis is crucial for lung formation but its regulatory mechanisms are not fully understood.
  • The roles of Lin28a and Lin28b paralogs in development are being investigated.

Purpose of the Study:

  • To investigate the function of Lin28a and Lin28b in lung branching morphogenesis.
  • To determine the stage-specific roles of Lin28 paralogs in post-transcriptional regulation during organogenesis.

Main Methods:

  • Studied the effects of Lin28a/b dysregulation on lung development in model organisms.
  • Analyzed the regulation of messenger RNAs (mRNAs) and microRNAs (miRNAs) by Lin28 paralogs.
  • Investigated the interaction between Lin28a and Sox9 in bypassing developmental defects.

Main Results:

  • Dysregulation of Lin28a/b causes abnormal branching morphogenesis in the lung and other organs.
  • Lin28 paralogs primarily control mRNA processing during early lung organogenesis.
  • Key target mRNAs include Sox2, Sox9, and Etv5, which are vital for lung development.
  • Functional interaction between Lin28a and Sox9 can rescue branching defects in Lin28a/b mutants.

Conclusions:

  • Lin28a and Lin28b are critical regulators of early embryonic lung development.
  • The timing of post-transcriptional regulation of both mRNAs and miRNAs by Lin28 paralogs is crucial for distinct stages of organogenesis.