Related Experiment Video
Updated: Oct 27, 2025

08:20
Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
Published on: March 28, 2016
8.1K
Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides
Lequn Geng1, Jiaqi Shen1, Wenjing Wang1
1Life Sciences Institute, University of Michigan, Ann Arbor, Michigan, USA. wenjwang@umich.edu and Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA.
Summary
Researchers engineered a light-sensing protein, AsLOV2, for precise control of peptides using circular permutation. This advancement enhances optogenetic tools for biological research.
Area of Science:
- Biochemistry
- Molecular Biology
- Optogenetics
Background:
- AsLOV2 is a widely utilized light-sensing protein.
- Optogenetic tools enable light-controlled biological processes.
- Controlling peptide function with light requires advanced protein engineering.
Purpose of the Study:
- To engineer a circularly permuted AsLOV2 (cpAsLOV2) for photoswitchable C-terminus control.
- To evaluate cpAsLOV2's efficacy alone and in combination with wild-type AsLOV2.
- To expand the toolkit for optogenetic engineering.
Main Methods:
- Circular permutation of the AsLOV2 protein.
- Characterization of the photoswitchable properties of cpAsLOV2.
- Demonstration of enhanced peptide caging using cpAsLOV2.
Main Results:
- Successfully created a circularly permuted AsLOV2 variant.
- Demonstrated photoswitchable control over peptide C-termini.
- Showed improved caging efficiency with cpAsLOV2, individually and combined with wild-type AsLOV2.
Conclusions:
- Circularly permuted AsLOV2 provides novel photoswitchable control.
- This engineered protein expands capabilities in optogenetic tool development.
- cpAsLOV2 offers enhanced caging for precise biological manipulation.

