Algoriphagus pacificus sp. nov. and Algoriphagus oliviformis sp. nov., isolated from a mariculture fishpond

Two Gram-stain-negative, catalase-positive, oxidase-negative, rod-shaped, non-flagellated, non-spore-forming and non-motile strains (YJ13C^T and H41^T) were isolated from a mariculture fishpond in PR China. Comparisons based on 16S rRNA gene sequences indicated that YJ13C^T and H41^T shared 16S rRNA gene sequences similarities between 92.6 and 99.2 % with species of the genus Algoriphagus. YJ13C^T only shared 93.8 % 16S rRNA gene sequence similarity with H41^T. The reconstructed phylogenetic and phylogenomic trees indicated that YJ13C^T and H41^T clustered closely with species of the genus Algoriphagus. The calculated pairwise orthologous average nucleotide identity with usearch (OrthoANIu) values between strains YJ13C^T and H41^T and other related strains were all less than 79.5 %. The OrthoANIu value between YJ13C^T and H41^T was only 69.9 %. MK-7 was the predominant respiratory quinone of YJ13C^T and H41^T and their major cellular fatty acids contained iso-C_15 : 0, C_16 : 1 ω7c and C_17 : 1 ω9c. The polar lipids profiles of YJ13C^T and H41^T consisted of phosphatidylethanolamine and several kinds of unidentified lipids. Combining the above descriptions, strains YJ13C^T and H41^T represent two distinct novel species of the genus Algoriphagus, for which the names Algoriphagus pacificus sp. nov. (type strain YJ13C^T=GDMCC 1.2178^T=KCTC 82450^T) and Algoriphagus oliviformis sp. nov. (type strain H41^T=GDMCC 1.2179^T=KCTC 82451^T) are proposed.