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Updated: Oct 27, 2025

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
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Genomic insights into methicillin-resistant Staphylococcus pseudintermedius isolates from dogs and humans of the same

Nathita Phumthanakorn1,2,3, Sybille Schwendener2, Valentina Donà2

  • 1Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.

Plos One
|July 22, 2021
PubMed

Insights

Genomic analysis reveals Methicillin-resistant Staphylococcus pseudintermedius (MRSP) from dogs and humans share similarities but exhibit distinct adaptations. ST45 MRSP strains show high genomic diversity, suggesting successful host adaptation.

Area of Science:

  • Veterinary Microbiology
  • Genomics
  • Zoonotic Diseases

Background:

  • Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is a significant opportunistic pathogen in dogs, with occasional human infections.
  • Understanding MRSP adaptation to human hosts is crucial, yet whole genome data from human isolates remain limited.

Purpose of the Study:

  • To compare the genomes of MRSP isolates from dogs and humans within the same sequence types (STs).
  • To investigate genomic similarities and differences, including prophage and pathogenicity island content, between human and canine MRSP.

Main Methods:

  • Comparative genomic analysis of three dog-human MRSP isolate pairs (ST45, ST112, ST181).
  • Identification and characterization of prophages and pathogenicity islands.
  • PCR screening for specific genetic elements in additional MRSP isolates.
  • Analysis of single nucleotide polymorphisms (SNPs) in core genomes.

Main Results:

  • Prophages associated with leucocidin genes were found in human MRSP, with remnants in dog MRSP.
  • A novel pathogenicity island, SpPI-181, was identified in ST181 isolates.
  • ST45 MRSP strains exhibited the highest core genome SNP count (3,612), indicating significant genomic diversity and potential for host adaptation.

Conclusions:

  • MRSP isolates from dogs and humans display host-specific genomic features and varying degrees of adaptation.
  • Genomic diversity in ST45 MRSP suggests successful adaptation and potential for broader host colonization.
  • This study provides valuable genomic insights into MRSP host adaptation and zoonotic potential.

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