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Assays for the Identification of Novel Antivirals against Bluetongue Virus
Published on: October 11, 2013
New antitumor antibiotic, LL-D05139 beta. Fermentation, isolation, structure determination and biological activities
M D Lee1, A A Fantini, N A Kuck
1American Cyanamid Co., Medical Research Division, Lederle Laboratories, Pearl River, New York 10965.
The Journal of Antibiotics
|December 1, 1987
Summary
Researchers discovered alanylazaserine (LL-D05139 beta) from Glycomyces harbinensis. This novel compound shows enhanced antibacterial and biochemical induction activities compared to azaserine, with similar antitumor effects.
Area of Science:
- Microbiology
- Natural Product Chemistry
- Biochemistry
Background:
- The bacterium Glycomyces harbinensis produces bioactive compounds.
- Azaserine is a known bioactive molecule with potential therapeutic applications.
Purpose of the Study:
- To isolate and characterize a novel complex (LL-D05139) from Glycomyces harbinensis.
- To determine the structure and biological activities of LL-D05139 beta.
Main Methods:
- Fermentation of Glycomyces harbinensis and isolation of the LL-D05139 complex.
- Development of a chemically defined medium to optimize LL-D05139 beta production.
- Purification using adsorption chromatography and microcrystalline cellulose chromatography.
- Structural elucidation using acid hydrolysis, GC analysis, Edman degradation, IR, UV, NMR, and MS.
Main Results:
- The LL-D05139 complex contains LL-D05139 beta and azaserine.
- The structure of LL-D05139 beta was determined to be alanylazaserine.
- LL-D05139 beta exhibited superior antibacterial and biochemical induction assay activities compared to azaserine.
- Both LL-D05139 beta and azaserine displayed comparable antitumor activities.
Conclusions:
- Alanylazaserine (LL-D05139 beta) is a novel antibiotic produced by Glycomyces harbinensis.
- LL-D05139 beta possesses enhanced biological activities, suggesting its potential as a therapeutic agent.
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