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Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
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Conversion of brewers' spent grain into proteinaceous animal feed using solid state fermentation
Christos Eliopoulos1,2, Dimitrios Arapoglou3, Nikos Chorianopoulos1
1Institute of Technology of Agricultural Products, ELGO DIMITRA, Sof. Venizelou 1, 14123, Athens, Greece.
Summary
Solid state fermentation (SSF) of brewers' spent grain (BSG) using Pleurotus ostreatus significantly enhanced its nutritional value. This bioprocess increases protein and beta-glucan content, creating a valuable animal feed and reducing brewing industry waste.
Area of Science:
- Biotechnology
- Agricultural Science
- Food Science
Background:
- Brewers' spent grain (BSG) is a major byproduct of beer production, with over 30 million tons generated globally each year.
- The valorization of BSG is crucial for reducing the environmental impact of the brewing industry and addressing the demand for sustainable animal feed.
- Current methods for BSG utilization often fall short of maximizing its nutritional potential for animal feed applications.
Purpose of the Study:
- To investigate the application of solid state fermentation (SSF) to improve the nutritional quality of brewers' spent grain (BSG).
- To evaluate the efficacy of Pleurotus ostreatus in enhancing the protein and beta-glucan content of BSG.
- To assess the potential of SSF-treated BSG as a high-value ingredient for animal feed.
Main Methods:
- Solid state fermentation (SSF) was employed using brewers' spent grain (BSG) as the substrate.
- The edible fungus Pleurotus ostreatus was utilized to initiate the SSF process.
- Key nutritional parameters, including protein content, beta-glucans, and cellulose, were analyzed before and after fermentation.
Main Results:
- The SSF process resulted in a significant 49.49% increase in the protein content of BSG.
- A remarkable 10-fold increase in 1,3-1,6 beta-glucans was observed after fermentation.
- Cellulose content was reduced by 11.42%, indicating improved digestibility.
Conclusions:
- Fungi-initiated SSF is a viable bioprocess for upgrading the nutritional profile of brewers' spent grain (BSG).
- Pleurotus ostreatus effectively enhances protein and beta-glucan levels in BSG, creating a more valuable animal feed.
- This approach offers a sustainable solution for managing brewing byproducts and producing nutrient-rich animal feed, addressing feed shortages.
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