Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells Using miRNA Switches
Kenji Miki1, Hirohide Saito2, Yoshinori Yoshida3
1Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|July 24, 2021
Abstract:
The most common method for isolating cells of interest is an antibody method that recognizes cell surface antigens. However, specific surface antigens for many cell types have not been identified. We have developed the microRNA (miRNA)-responsive synthetic mRNA systems (miRNA switches), which isolate target cells based on endogenous miRNA activity. In this chapter, we describe protocols for isolating human pluripotent stem cell (hPSC)-derived cardiomyocytes using miRNA switches with or without cell sorting.
