Effects of femtosecond laser-assisted trephination on donor tissue in liquid interface as compared to applanated...

To evaluate the effects of femtosecond laser-assisted keratoplasty using a liquid patient interface (L-PI) as opposed to an applanated interface (A-PI) on graft quality and functionality markers.

Pressure measurements during femtosecond laser-assisted trephination were performed using two groups of 10 porcine eyes. Trephination was performed either in an L-PI or in an A-PI setting. Pressure sensor needles placed intravitreally continuously recorded intraocular pressure during trephination. Twenty paired human donor eyes were used to test the morphological quality of donor tissue after trephination in L-PI and A-PI settings. Optical coherence tomography (OCT) scans were performed before and after trephination. Images were processed using ImageJ and pixel² .

During trephination, pressure measurements with an L-PI were significantly lower than with an A-PI (p = 0.0121). Mean pressure during trephination was 78.1 mmHg ± 37.6 mmHg with L-PI and 188.6 mmHg ± 17.7 mmHg with A-PI. Trephination in A-PI produced a significantly larger increase (p < 0.00001) in donor pachymetry than trephination in L-PI. Significantly lower areas of Descemet folds were achieved in L-PI trephination than in A-PI trephination (p < 0.01). There was no significant difference in circularity between A-PI and L-PI (p = 0.27). Total time required for trephination was comparable between L-PI and A-PI (p = 0.45). Time taken to reach working vacuum was achieved significantly more quickly in L-PI (p < 0.05).

Femtosecond laser-assisted L-PI keratoplasty appears to be a promising method to decrease stress to donor and recipient tissue during femtosecond laser-assisted trephination. Results showed favourable donor tissue morphology markers after L-PI trephination.