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Evaluation of the eazyplex® EHEC complete (Amplex) assay for the differentiation of stx1 and stx2 positivity in stool
Hannes Bjarki Vigfússon1, Theresa Ennefors1, Torbjörn Kjerstadius2
1Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, 701 85 Örebro, Sweden.
Journal of Microbiological Methods
|July 26, 2021
Abstract:
The performance of the eazyplex® EHEC complete (Amplex) for the detection of Shiga toxin genes in stool samples was evaluated. The assay performed well in distinguishing between stx1 and stx2 but suboptimal sensitivity may limit its use to complementary testing rather than primary diagnosis of Shiga toxin-producing Escherichia coli infections.
Keywords:
Enterohemorrhagic Escherichia coliLoop-mediated amplificationShiga toxinShiga toxin-producing Escherichia coli
