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Published on: November 29, 2019
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Quantification of intracellular ACBP/DBI levels
Valentina Sica1, Isabelle Martins2, Federico Pietrocola3
1Department of Experimental and Health Sciences, Pompeu Fabra University (UPF), Barcelona, Spain; Center for Networked Biomedical Research in Neurodegenerative Diseases (CIBERNED), Madrid, Spain.
Methods in Cell Biology
|July 27, 2021
Summary
Acyl-CoA binding protein (ACBP) secretion via autophagy regulates lipid metabolism. Inhibited autophagy increases ACBP, promoting lipid anabolism and linking obesity to impaired autophagy.
Area of Science:
- Biochemistry
- Cell Biology
- Metabolism
Background:
- Acyl-CoA binding protein (ACBP), also known as diazepam-binding inhibitor (DBI), is a secreted protein involved in lipid metabolism.
- ACBP/DBI levels and localization influence cellular lipid metabolism.
- Autophagy regulates ACBP/DBI secretion, impacting lipid catabolism and anabolism.
Purpose of the Study:
- To detail protocols for quantifying ACBP/DBI levels.
- To indirectly assess autophagy-dependent ACBP/DBI release.
- To elucidate the role of ACBP/DBI in autophagy-mediated lipid metabolism regulation.
Main Methods:
- Immunofluorescence assays for ACBP/DBI detection.
- Image flow cytometry for ACBP/DBI quantification.
- Immunoblot techniques to measure ACBP/DBI levels.
Main Results:
- Established protocols allow for the measurement of intracellular ACBP/DBI levels.
- Quantification of ACBP/DBI indirectly reflects its autophagy-dependent release.
- ACBP/DBI levels correlate with lipid metabolism regulation.
Conclusions:
- Autophagy-dependent secretion of ACBP/DBI is a key regulator of lipid metabolism.
- Altered autophagy impacts intracellular ACBP/DBI levels, influencing lipid anabolism and catabolism.
- The described methods facilitate the study of ACBP/DBI in various physiological and pathological conditions.
