Related Experiment Video
Updated: Oct 26, 2025
06:24
Fertility Sparing Procedure using Carbon Dioxide Fiber Laser Vaporization of Ovarian Endometrioma
Published on: July 6, 2022
5.1K
Single live birth derived from conjoined oocytes using laser-cutting technique: a case report
Qin Wang1, Ashley Ulker2, Hongfeng Wang2
1Department of Reproductive Medicine, Affiliated Hospital of Jining Medical University, Shandong, China.
Zygote (Cambridge, England)
|July 27, 2021
Summary
Conjoined oocytes, a rare IVF finding, can now be successfully utilized. A novel protocol using laser-cutting and ICSI enables normal pregnancy from these oocytes, benefiting patients with limited egg supply.
Area of Science:
- Reproductive biology
- Assisted reproductive technology
Background:
- Conjoined oocytes are rare, occurring in 0.3% of retrieved human oocytes.
- This phenomenon differs from single oocyte development and can lead to dizygotic twins or mosaicism.
- Current management of conjoined oocytes in in vitro fertilization (IVF) varies due to limited evidence.
Observation:
- This report introduces a new protocol for managing conjoined oocytes using intracytoplasmic sperm injection (ICSI), laser-cutting, and next-generation sequencing (NGS).
- Case 1: Conjoined oocytes successfully penetrated their shared zona pellucida (ZP) by Day 6.
- Case 2: A patient underwent successful embryo transfer after laser-cutting separation of conjoined oocytes, ICSI, and NGS testing.
Findings:
- The described protocol facilitated a successful pregnancy and live birth of a healthy female infant from conjoined oocytes.
- Laser-cutting separation of conjoined oocytes is a viable technique for achieving normal pregnancy.
Implications:
- This protocol offers a potential solution for patients with a limited oocyte supply undergoing IVF.
- It provides a method to potentially salvage and utilize conjoined oocytes, improving IVF success rates.
