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Single live birth derived from conjoined oocytes using laser-cutting technique: a case report.

Qin Wang1, Ashley Ulker2, Hongfeng Wang2

  • 1Department of Reproductive Medicine, Affiliated Hospital of Jining Medical University, Shandong, China.

Zygote (Cambridge, England)
|July 27, 2021
PubMed
Summary

Conjoined oocytes, a rare IVF finding, can now be successfully utilized. A novel protocol using laser-cutting and ICSI enables normal pregnancy from these oocytes, benefiting patients with limited egg supply.

Keywords:
Conjoined oocytesICSILaser-cuttingNext-generation sequencingSingle pregnancy

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Area of Science:

  • Reproductive biology
  • Assisted reproductive technology

Background:

  • Conjoined oocytes are rare, occurring in 0.3% of retrieved human oocytes.
  • This phenomenon differs from single oocyte development and can lead to dizygotic twins or mosaicism.
  • Current management of conjoined oocytes in in vitro fertilization (IVF) varies due to limited evidence.

Observation:

  • This report introduces a new protocol for managing conjoined oocytes using intracytoplasmic sperm injection (ICSI), laser-cutting, and next-generation sequencing (NGS).
  • Case 1: Conjoined oocytes successfully penetrated their shared zona pellucida (ZP) by Day 6.
  • Case 2: A patient underwent successful embryo transfer after laser-cutting separation of conjoined oocytes, ICSI, and NGS testing.

Findings:

  • The described protocol facilitated a successful pregnancy and live birth of a healthy female infant from conjoined oocytes.
  • Laser-cutting separation of conjoined oocytes is a viable technique for achieving normal pregnancy.

Implications:

  • This protocol offers a potential solution for patients with a limited oocyte supply undergoing IVF.
  • It provides a method to potentially salvage and utilize conjoined oocytes, improving IVF success rates.