Facial Skin Microbiota-Mediated Host Response to Pollution Stress Revealed by Microbiome Networks of Individual

Urban living has been reported to cause various skin disorders. As an integral part of the skin barrier, the skin microbiome is among the key factors associated with urbanization-related skin alterations. The role of skin microbiome in mediating the effect of urban stressors (e.g., air pollutants) on skin physiology is not well understood. We generated 16S sequencing data and constructed a microbiome network of individual (MNI) to analyze the effect of pollution stressors on the microbiome network and its downstream mediation effect on skin physiology in a personalized manner. In particular, we found that the connectivity and fragility of MNIs significantly mediated the adverse effects of air pollution on skin health, and a smoking lifestyle deepened the negative effects of pollution stress on facial skin microbiota. This is the first study that describes the mediation effect of the microbiome network on the skin's physiological response toward environmental factors as revealed by our newly developed MNI approach and conditional process analysis. IMPORTANCE The association between the skin microbiome and skin health has been widely reported. However, the role of the skin microbiome in mediating skin physiology remains a challenging and yet priority subject in the field. Through developing a novel MNI method followed by mediation analysis, we characterized the network signature of the skin microbiome at an individual level and revealed the role of the skin microbiome in mediating the skin's responses toward environmental stressors. Our findings may shed new light on microbiome functions in skin health and lay the foundation for the design of a microbiome-based intervention strategy in the future.