Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Efficacy of saline tract sealing after CT-Guided lung biopsy: a systematic review and meta-analysis.

Clinical radiology·2026

Same author

Functionalization of Photocrosslinkable GelMA Hydrogel With Metal Oxides for Direct Pulp Capping.

BioMed research international·2026

Same author

Biological, antimicrobial, and apical tissue dissolution effects of ambroxol hydrochloride as an irrigant for regenerative endodontics: an in vitro study.

BMC oral health·2026

Same author

Impact of EDTA Formulations and Activation Methods on Stem Cell Response, Antimicrobial Activity and Dentine Integrity: An In Vitro Study.

Australian endodontic journal : the journal of the Australian Society of Endodontology Inc·2026

Same author

Decellularized bone matrix-enriched 3D-printed GelMA scaffold as a cell-homing platform: analysis using an artificial pulp chamber model.

Clinical oral investigations·2026

Same author

Cellular and molecular immunomodulatory potential of red wine polyphenols in apical periodontitis.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2026

Same journal

Gold Nanoparticles Enhance the Antibacterial and Osteogenic Properties of Polyetheretherketone.

Journal of dental research·2026

Same journal

Periodontitis-Aggravated Diabetic Kidney Disease with Altered Glycolysis.

Journal of dental research·2026

Same journal

Response to Letter to Editor: "Estimating the Individualized Effect of Tooth Extraction before Radiotherapy on Osteoradionecrosis Using Causal Machine Learning".

Journal of dental research·2026

Same journal

Reorienting Oral Health Promotion through Systems Thinking.

Journal of dental research·2026

Same journal

<i>Porphyromonas gingivalis</i>-Induced NETs Mediate Neuroinflammation via TLR4 Activation.

Journal of dental research·2026

Same journal

Oral Burden of Sjögren Disease: A Systematic Review and Meta-analysis.

Journal of dental research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 26, 2025

Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

Published on: March 10, 2023

Chitosan-Calcium-Simvastatin Scaffold as an Inductive Cell-Free Platform.

D G Soares¹, E A F Bordini², E S Bronze-Uhle¹

¹Department of Operative Dentistry, Endodontics and Dental Materials, São Paulo University-USP, Bauru School of Dentistry, Bauru, SP, Brazil.

Journal of Dental Research

|July 28, 2021

Summary

This summary is machine-generated.

This study developed a simvastatin-releasing chitosan-calcium-hydroxide scaffold to enhance dentin regeneration. The scaffold successfully promoted dental pulp cell differentiation and mineralization, offering a promising strategy for regenerative dentistry.

Keywords:

biocompatible materials cell culture techniques dental pulp dentin regenerative medicine tissue engineering

More Related Videos

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

Published on: June 1, 2012

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

Published on: June 13, 2018

Related Experiment Videos

Last Updated: Oct 26, 2025

Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

Published on: March 10, 2023

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

Published on: June 1, 2012

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

Published on: June 13, 2018

Area of Science:

Biomaterials Science
Regenerative Dentistry
Tissue Engineering

Background:

Current regenerative dentistry strategies aim to develop biomaterials for dentin regeneration using biopolymers and bioactive compounds.
Chitosan-calcium-hydroxide (CH-Ca) scaffolds are being investigated for their potential in dental pulp cell modulation.
Simvastatin (SV) is a bioactive compound with potential to enhance regenerative processes.

Purpose of the Study:

To assess the bioactive potential of a simvastatin (SV)-releasing chitosan-calcium-hydroxide (CH-Ca) scaffold for dentin regeneration.
To evaluate the scaffold's ability to modulate dental pulp cells (DPCs) and promote odontoblastic differentiation.
To investigate the in vitro and in vivo performance of the CH-Ca-SV scaffold in a dentin regeneration model.

Main Methods:

Characterization of SV-incorporated CH-Ca scaffolds using Fourier-transform infrared spectroscopy.
Assessment of DPC cytocompatibility, proliferation, and odontoblastic differentiation on scaffolds in a dentin microenvironment.
In vitro simulation of internal pressure and in vivo implantation in rat calvaria defects.

Main Results:

Fourier-transform infrared spectroscopy confirmed the incorporation of calcium (Ca) and SV into the scaffold structure.
SV-releasing scaffolds supported DPC proliferation and enhanced odontoblastic differentiation, evidenced by marker overexpression and mineralized matrix deposition.
In vivo studies demonstrated intense mineralization within the CH-Ca-SV scaffold, indicating successful new tissue formation.

Conclusions:

The CH-Ca-SV scaffold effectively induces DPC differentiation into a highly mineralizing phenotype in the presence of dentin.
The scaffold creates a microenvironment that attracts pulp cells and promotes odontoblastic marker expression, supporting a cell-homing strategy.
This biomaterial presents a promising approach for enhancing dentin regeneration through modulated resident cell activity.