Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

3.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.3K
MicroRNAs01:22

MicroRNAs

22.5K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of the SARS-CoV-2 genome packaging signal in the nsp12-coding region.

Nature communications·2026
Same author

Directed evolution of compact RNA-guided nucleases for enhanced activity in mammalian cells.

Genome biology·2026
Same author

Structural basis for chaperone-guided assembly of RNA-induced silencing complex.

Nature·2026
Same author

Pool-packaged AAV libraries exhibit extensive length-dependent and homology-dependent chimerism.

Nature biotechnology·2026
Same author

CEPI Workshop Report: Applying Disease X Vaccine Library and Knowledge Base Approaches to Severe Fever with Thrombocytopenia Syndrome (SFTS).

Vaccines·2026
Same author

Multi-omics Differential Inference for Functional Interpretation (MoDIFI): A Statistical Framework to Prioritize Cell Lines for Neurodevelopmental Variants.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Oct 26, 2025

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

7.6K

A quantitative map of human primary microRNA processing sites.

Kijun Kim1, S Chan Baek1, Young-Yoon Lee1

  • 1Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea; School of Biological Sciences, Seoul National University, Seoul 08826, Korea.

Molecular Cell
|July 28, 2021
PubMed
Summary

Most human microRNAs (miRNAs) are not processed by DROSHA as expected. This study identifies DROSHA-dependent miRNAs and reveals alternative processing events, impacting miRNA regulation.

Keywords:
DGCR8DROSHAMicroprocessorSRSF3microRNApri-miRNA processing

More Related Videos

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.1K
Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
10:55

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Published on: March 8, 2019

8.4K

Related Experiment Videos

Last Updated: Oct 26, 2025

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

7.6K
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.1K
Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
10:55

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Published on: March 8, 2019

8.4K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • MicroRNA (miRNA) biogenesis is crucial for gene regulation.
  • Canonical miRNA maturation begins with DROSHA cleavage of primary miRNA (pri-miRNA) transcripts.
  • The precise cleavage sites and DROSHA dependence for most human pri-miRNAs remain uncharacterized.

Purpose of the Study:

  • To comprehensively profile in vitro processing of all human pri-miRNAs.
  • To classify pri-miRNAs based on DROSHA dependence and map cleavage sites.
  • To identify factors and mechanisms regulating pri-miRNA processing.

Main Methods:

  • In vitro processing assays on a full set of human pri-miRNAs (miRBase version 21).
  • High-throughput sequencing of processed transcripts.
  • Bioinformatic analysis to map cleavage sites and assess processing efficiency.
  • Identification of cis-regulatory elements and auxiliary factors.

Main Results:

  • Only 758 out of over 1,800 annotated human pri-miRNAs are confidently processed by DROSHA.
  • The majority of annotated pri-miRNAs may be non-canonical or incorrectly annotated.
  • Widespread alternative and unproductive processing events (e.g., 'nick' or 'inverse' processing) were observed.
  • SRSF3 was identified as an auxiliary factor that modulates alternative processing and suppresses unproductive cleavage.

Conclusions:

  • A significant portion of annotated human miRNA loci may not follow canonical DROSHA processing.
  • Detailed mapping of DROSHA cleavage sites provides insights into miRNA processing regulation.
  • Understanding alternative and unproductive processing is key to accurate miRNA annotation and functional studies.
  • The developed methods and data enable systematic investigation of miRNA biogenesis regulation.