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Updated: Oct 26, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
A draft reference assembly of the Psilocybe cubensis genome
Kevin McKernan1, Liam T Kane1, Seth Crawford2
1R&D, Medicinal Genomics, Beverly, Mass, 01915, USA.
Abstract:
We describe the use of high-fidelity single molecule sequencing to assemble the genome of the psychoactive Psilocybe cubensis mushroom. The genome is 46.6Mb, 46% GC, and in 32 contigs with an N50 of 3.3Mb. The BUSCO completeness scores are 97.6% with 1.2% duplicates. The Psilocybin synthesis cluster exists in a single 3.2Mb contig. The dataset is available from NCBI BioProject with accessions PRJNA687911 and PRJNA700437.
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