Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Computer-Assisted Hapten Design for High-Performance Recombinant Antibody against Napropamide: Molecular Recognition Insight and Dual T-Line Lateral Flow Immunoassay Development.

Analytical chemistry·2026
Same author

Spin-controlled enantioselective near-infrared photocatalysis with chiral MgO/Co<sub>3</sub>O<sub>4</sub> nanoparticles.

Nature communications·2026
Same author

Guiding point-of-care therapeutic drug monitoring through structure-toxicity principles.

Chemical science·2026
Same author

Computer-Aided Rational Hapten Design for Broad-Spectrum Monoclonal Antibody Development against Anthraquinones and Its Application in Lateral Flow Immunoassay.

Analytical chemistry·2026
Same author

Fluorescence Polarization Immunoassay with Modulated Selectivity for Effective Detection of the Agrochemical 4-Chlorophenoxyacetic Acid.

Biosensors·2026
Same author

An ultrasensitive visual detection platform for forchlorfenuron: from simulation-assisted hapten and antibody production to double-T immunochromatographic assay application.

Journal of materials chemistry. B·2026

Related Experiment Video

Updated: Oct 26, 2025

Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

13.7K

Single-Molecule Binding Assay Using Nanopores and Dimeric NP Conjugates.

Ren Ren1, Maozhong Sun2, Pratibha Goel1

  • 1Department of Chemistry, Molecular Science Research Hub, Imperial College London, White City Campus, 82 Wood Lane, London, W12 0BZ, UK.

Advanced Materials (Deerfield Beach, Fla.)
|July 29, 2021
PubMed
Summary
This summary is machine-generated.

New nanoparticle probes enable highly sensitive, single-molecule detection of biomarkers in biological fluids. This breakthrough advances disease diagnosis and monitoring using nanopore sensing technology without complex sample preparation.

Keywords:
diagnosticsmolecular probesnanopore sensingscreeningself-assemblysingle-molecule detection

More Related Videos

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles
08:31

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

Published on: March 20, 2019

7.7K
Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

8.0K

Related Experiment Videos

Last Updated: Oct 26, 2025

Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

13.7K
A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles
08:31

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

Published on: March 20, 2019

7.7K
Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

8.0K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Single-molecule detection of biomarkers is crucial for disease diagnosis and monitoring.
  • Nanopore sensing offers a promising platform for biomarker analysis but faces challenges in selectivity and differentiating bound from unbound targets.
  • Current methods often require sample processing or amplification, limiting their efficiency.

Purpose of the Study:

  • To develop highly sensitive and selective molecular probes for single-molecule biomarker detection using nanopore sensing.
  • To create nanoparticle-based probes that self-assemble and dimerize upon binding to biological targets.
  • To demonstrate the utility of these probes for detecting analytes like antigens, antibodies, and microRNAs.

Main Methods:

  • Design and synthesis of nanoparticle (NP)-based molecular probes.
  • Utilizing self-assembly and dimerization of NPs upon target binding.
  • Employing single-molecule nanopore sensing to detect individual and paired NPs.
  • Application in antigen/antibody detection and microRNA sequence analysis.

Main Results:

  • Successfully designed and fabricated nanoparticle probes that self-assemble and dimerize upon target binding.
  • Demonstrated the ability to resolve and detect both single and paired NPs at the single-molecule level using nanopore sensing.
  • Validated the probes for specific applications including antigen/antibody detection and microRNA analysis.

Conclusions:

  • The developed nanoparticle probes offer a novel strategy for highly sensitive and selective molecular detection.
  • This technology enables single-molecule analysis in biological fluids, potentially transforming disease diagnosis and screening.
  • The approach minimizes sample processing and amplification needs, requiring only minimal sample volumes.