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Using RNA-Seq to Explore the Hub Genes in the Trigeminal Root Entry Zone of Rats by Compression Injury.

Ran Tao1, Feng Huang1, Kun Lin2

  • 1Department of Human Anatomy, Laboratory of Clinical Applied Anatomy, School of Basic Medical Science, Fujian Medical University, Fuzhou, China.

Pain Physician
|July 29, 2021
PubMed
Summary

This study identified six key genes in the trigeminal nerve root entry zone associated with trigeminal neuralgia (TN) in a rat model. These findings offer potential new targets for understanding and treating TN.

Keywords:
RNA-seqcompression injuryhub generattranscriptometrigeminal neuralgiatrigeminal root entry zoneAnimal model

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Area of Science:

  • Neuroscience
  • Genomics
  • Molecular Biology

Background:

  • Trigeminal neuralgia (TN) is primarily caused by microvascular compression of the trigeminal nerve root entry zone (TREZ).
  • Understanding the pathogenesis of TN is crucial for developing effective treatments.

Purpose of the Study:

  • To identify hub genes within the TREZ of a TN animal model to elucidate disease pathogenesis.
  • To investigate gene expression changes associated with chronic compression of the trigeminal nerve root.

Main Methods:

  • A controlled animal trial using a TN rat model induced by chronic compression of the trigeminal nerve root (CCT).
  • RNA sequencing (RNA-Seq) was employed to analyze gene expression in the TREZ.
  • Bioinformatic analyses including KEGG, GO, and PPI were performed on differentially expressed genes (DEGs), alongside Gene Set Enrichment Analysis (GSEA).
  • Reverse transcription real-time polymerase chain reaction (RT-qPCR) validated RNA-Seq findings.

Main Results:

  • RNA-Seq identified 352 upregulated and 59 downregulated DEGs in the TN group 21 days post-operation.
  • KEGG analysis suggested involvement of "neuroactive ligand receptor interaction" and "cytokine cytokine receptor interaction" pathways.
  • GO analysis highlighted "regulation of signaling receptor activity," "chemokine activity," and "carbohydrate binding" as potentially related to TN pathogenesis.
  • RT-qPCR confirmed the reliability of the transcriptome sequencing results.

Conclusions:

  • Six hub genes in the TREZ were identified as closely related to the TN animal model.
  • These identified hub genes represent potential targets for further research into the pathogenesis of TN.
  • The study provides a foundation for exploring novel therapeutic strategies for trigeminal neuralgia.