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Updated: Oct 26, 2025

Live Cell Imaging of Chromosome Segregation During Mitosis
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Live-cell imaging probes to track chromatin modification dynamics.

Yuko Sato1,2, Masaru Nakao2, Hiroshi Kimura1,2

  • 1Cell Biology Center, Institute of Innovative Research, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8503, Japan.

Microscopy (Oxford, England)
|July 30, 2021
PubMed
Summary

Live-cell probes enable visualization of dynamic chromatin modifications, offering new ways to study genome function and screen epigenome drugs.

Keywords:
DNA methylationepigeneticsfluorescence microscopyhistone modificationintrabodieslive-cell imaging

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Cell Biology

Background:

  • Chromatin organization is crucial for genome function.
  • Epigenetic modifications like DNA methylation and histone modifications regulate chromatin.
  • Dynamic chromatin changes are not well understood.

Purpose of the Study:

  • To review methods for visualizing dynamic chromatin modifications in living cells.
  • To highlight the potential of live-cell imaging for understanding chromatin regulation.
  • To discuss applications in drug screening for epigenome-related diseases.

Main Methods:

  • Development of live-cell probes using fluorescent proteins and genome editing.
  • Engineering of probes based on modification-binding modules (e.g., chromodomains, antibody fragments).
  • Utilizing Förster resonance energy transfer (FRET), fluorescence complementation, and luciferase chemiluminescence for detection.

Main Results:

  • Various probes can effectively track specific chromatin modifications in real-time.
  • These probes can visualize dynamic chromatin regulation without disrupting cellular processes.
  • Successful development of sensors for intramolecular conformational changes.

Conclusions:

  • Live-cell imaging of chromatin modifications is a powerful tool.
  • This technology advances the study of dynamic epigenomic regulation.
  • Applications include screening for novel epigenome drugs.