Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Scanning microscopic observations on dental caries.

S J Jones1, A Boyde

  • 1Department of Anatomy and Developmental Biology, University College London, England.

Scanning Microscopy
|December 1, 1987
PubMed
Summary

Advanced imaging techniques reveal crucial changes in tooth structure during dental caries. These methods offer higher resolution for studying enamel, dentine, and cementum demineralization and microhardness variations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Topographically guided hierarchical mineralization.

Materials today. Bio·2021
Same author

The plate-to-rod transition in trabecular bone loss is elusive.

Royal Society open science·2021
Same author

Evaluation of laser ablation microtomy for correlative microscopy of hard tissues.

Journal of microscopy·2018
Same author

The relationship between the edge of the chick blastoderm and the vitelline membrane.

Wilhelm Roux' Archiv fur Entwicklungsmechanik der Organismen·2017
Same author

Multiscale alterations in bone matrix quality increased fragility in steroid induced osteoporosis.

Bone·2015
Same author

Calcium silicate cement-induced remineralisation of totally demineralised dentine in comparison with glass ionomer cement: tetracycline labelling and two-photon fluorescence microscopy.

Journal of microscopy·2014

Area of Science:

  • Dental research
  • Microscopy
  • Materials science

Background:

  • Dental caries involves complex structural changes in enamel, dentine, and cementum.
  • Previous methods for studying demineralization, like polarized light and microradiography, have limitations in resolution.
  • Understanding these changes is crucial for developing effective caries prevention and treatment strategies.

Purpose of the Study:

  • To investigate structural changes in tooth tissues during coronal and root caries.
  • To evaluate the effectiveness of advanced imaging techniques for caries research.
  • To characterize demineralization and microhardness variations in carious dental tissues.

Main Methods:

  • Scanning electron microscopy (SEM) with backscattered electron (BSE) imaging.
  • Confocal scanning optical microscopy (CSOM) with fluorescent dyes.
  • Microhardness testing using abrasive particle jet.
  • Tandem scanning reflected light microscopy (TSRLM).

Main Results:

  • BSE imaging revealed high-resolution details of density changes in enamel and dentine during caries.
  • Abrasive particle jet method effectively differentiated between carious and healthy dental tissues.
  • TSRLM provided insights into caries spread without extensive specimen preparation.
  • CSOM demonstrated pore volume distribution using fluorescent dyes.

Conclusions:

  • Advanced imaging techniques offer superior resolution and characterization of dental caries compared to traditional methods.
  • These techniques provide valuable insights into the microstructural alterations occurring in enamel, dentine, and cementum during caries progression.
  • The study highlights the potential of these methods for future research in dental caries and restorative dentistry.

Related Experiment Videos