RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells

Fluid shear stress (FSS) is an important mechanical force that regulates endothelial and vascular functions in the blood and lymphatic vasculature. RNA-sequencing (RNA-Seq), an emerging next-generation sequencing (NGS) technique, facilitates us to quantify the global gene expression levels and, thereby, provides us with a powerful tool to study the transcriptional reprograming in the cells. Here, we describe the RNA-Seq procedures and the subsequent bioinformatic analysis to understand the transcriptome regulation by FSS in lymphatic endothelial cells (LECs).