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Nanoparticles for In Vivo Lifetime Multiplexed Imaging.

Erving Ximendes1,2, Emma Martín Rodríguez1,3, Dirk H Ortgies1,2

  • 1Nanomaterials for BioImaging Group, Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS, Madrid, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|July 31, 2021
PubMed
Summary

Lifetime multiplexed imaging enables simultaneous labeling of biological structures using probes with identical spectra but distinct fluorescence lifetimes for in vivo imaging.

Keywords:
BioimagingNear-infrared imagingRare-earth-doped nanoparticles

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Area of Science:

  • Biophotonics and Imaging Science
  • Molecular and Cellular Biology
  • Medical Diagnostics

Background:

  • Multiplexed imaging is crucial for simultaneous visualization of multiple biological targets.
  • Traditional multiplexing often relies on distinct spectral properties, limiting probe choices.
  • Fluorescence lifetime offers an additional orthogonal contrast mechanism for biological imaging.

Purpose of the Study:

  • To introduce and define the principles of lifetime multiplexed imaging.
  • To highlight the requirements for implementing this technique in vivo.
  • To underscore the potential for quantitative information extraction in multichannel fluorescence imaging.

Main Methods:

  • Utilizes fluorescent probes with identical photoluminescence spectra.
  • Differentiates labeled structures based on distinct fluorescence lifetimes.
  • Requires near-infrared (NIR) fluorophores and time-resolved imaging systems.

Main Results:

  • Enables simultaneous labeling of different structures within a biological system.
  • Allows for quantitative data acquisition from multichannel in vivo fluorescence imaging.
  • Demonstrates the feasibility of extracting lifetime-based information.

Conclusions:

  • Lifetime multiplexed imaging provides a novel approach for advanced biological visualization.
  • The technique necessitates specific fluorophore properties (tunable lifetimes, NIR bands) and advanced imaging hardware.
  • Offers enhanced capabilities for in vivo quantitative analysis in complex biological samples.