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Highly-Multiplexed Tissue Imaging with Raman Dyes
Published on: April 21, 2022
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Multiplexed Raman Imaging in Tissues and Living Organisms
Travis M Shaffer1, Sanjiv S Gambhir2,3,4
1Department of Radiology, Stanford University, Stanford, CA, USA. trvsshaffer@gmail.com.
Methods in Molecular Biology (Clifton, N.J.)
|July 31, 2021
Summary
Surface-enhanced Raman scattering (SERS) nanoparticles offer sensitive in vivo imaging and tissue staining. A new library of SERS nanoparticles enables multiplexing applications with minimal background noise.
Area of Science:
- Nanotechnology
- Biomedical Imaging
- Spectroscopy
Background:
- Surface-enhanced Raman scattering (SERS) nanoparticles (NPs) are valuable tools for biological applications.
- Their high sensitivity and unique spectral fingerprints offer advantages over traditional optical imaging methods.
- Multiplexing capabilities are crucial for advanced diagnostics and research.
Purpose of the Study:
- To synthesize a novel library of SERS nanoparticles.
- To demonstrate the in vivo multiplexing application of these SERS NPs.
- To highlight the potential of SERS NPs in advanced imaging and diagnostics.
Main Methods:
- Synthesis of a SERS nanoparticle library.
- Characterization of SERS NP properties, including sensitivity and spectral uniqueness.
- In vivo testing of SERS NPs for multiplexing applications in imaging and tissue staining.
Main Results:
- Successful synthesis of a diverse SERS NP library.
- Demonstration of attomolar concentration detection limits.
- Successful in vivo multiplexing imaging and tissue staining using the SERS NP library.
- Minimal background signal achieved compared to other optical modalities.
Conclusions:
- The developed SERS NP library is suitable for sensitive in vivo multiplexing.
- SERS NPs provide a powerful platform for advanced biomedical imaging and diagnostics.
- This technology offers a unique molecular fingerprint for precise analysis.
