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Mangiferin Inhibits Human Lung Adenocarcinoma by Suppressing MiR-27b and MiR-92a
Xiao-Jv Chi1, Jian-Jun Meng2, Chun-Yu Lin1
1Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region 530021, China.
Abstract:
Lung adenocarcinoma (LUAD) is one of the most prevalent malignancies. However, its mechanism and therapeutic strategy remain to be clarified. Mangiferin is a flavonoid derived from the leaves of mango trees of the lacquer family that has many pharmacological and physiological effects. This research aimed to elucidate the biological effect of mangiferin in LUAD cell lines and clarify the in vitro mechanism of mangiferin. Mangiferin was shown to significantly restrain the proliferation of LUAD cells (A549, H1299, and H2030 cells) in a dose- and time-dependent manner. Furthermore, mangiferin was capable of stimulating apoptosis, and more cells were blocked in G1 and S phase in the mangiferin-treated cells than in those not treated with mangiferin. Microarrays and micro-RNA sequencing data suggested that there is a higher level of miR-27b and miR-92a in LUAD tissues than in non-LUAD tissues. Additional experiments indicated that mangiferin may be related to the downregulated levels of miR-92a and miR-27b. In conclusion, mangiferin likely regulates proliferation and apoptosis in LUAD cells by reducing the expression levels of miR-92a and miR-27b.
Insights
Mangiferin, a natural compound, inhibits lung adenocarcinoma (LUAD) cell growth and promotes apoptosis. It appears to achieve this by downregulating miR-92a and miR-27b expression in LUAD cells.
Area of Science:
- Oncology
- Molecular Biology
- Pharmacology
Background:
- Lung adenocarcinoma (LUAD) is a common cancer with unclear mechanisms and treatment strategies.
- Mangiferin, a flavonoid from mango leaves, possesses various pharmacological effects.
- Understanding mangiferin's role in LUAD is crucial for developing new therapies.
Purpose of the Study:
- To investigate the biological effects of mangiferin on LUAD cell lines.
- To elucidate the in vitro mechanism of mangiferin's action in LUAD.
- To explore the potential role of specific microRNAs in mangiferin's anti-cancer activity.
Main Methods:
- Mangiferin treatment of LUAD cell lines (A549, H1299, H2030).
- Cell proliferation assays and cell cycle analysis (G1/S phase).
- Microarray and micro-RNA sequencing to identify differentially expressed microRNAs.
Main Results:
- Mangiferin significantly inhibited LUAD cell proliferation in a dose- and time-dependent manner.
- Mangiferin induced apoptosis and caused cell cycle arrest at G1 and S phases.
- Mangiferin treatment correlated with downregulated levels of miR-92a and miR-27b, which are upregulated in LUAD tissues.
Conclusions:
- Mangiferin exhibits anti-proliferative and pro-apoptotic effects on LUAD cells.
- Mangiferin's mechanism involves the downregulation of miR-92a and miR-27b.
- Mangiferin shows potential as a therapeutic agent for lung adenocarcinoma.
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